The sensitivities and specificities of enzyme immunoassay (EIA) kits for the detection of antibodies to hepatitis C virus (HCV) had been in contrast in donor blood screening. A complete of 3308 donors had been examined utilizing Akvapast immunodiagnosticum (St. Petersburg), lot 12.
Anti-HCV antibodies had been detected in 43 (1.3%) topics. Blood sera of those donors had been moreover investigated utilizing different numerous Akvapast, Hepascan (Bioservice, Moscow), Blot-0.5 (Bioservice, Moscow), and immunoComb (France). The research demonstrated that not one of the brokers used will be acknowledged as ideally delicate and particular for the detection of anti-HCV antibodies.
Maximally delicate diagnostic kits ought to be most popular for the screening of donors of blood elements and for different mass surveys. Extremely-specific check techniques are advisable for profound examinations and diagnostic monitoring. The creation of a system of reference and skilled prognosis of hepatitis C stays a urgent job.
[Detection of the markers of herpes simplex virus and cytomegalovirus in newborns and infants].
A complete of 111 kids suspected for herpesvirus an infection had been examined. In blood and urine samples the infectious exercise of herpes simplex virus (HSV) and cytomegalovirus (CMV) was detected by the speedy tradition technique (RCM) and the presence of virus DNA–by the polymerase chain response (PCR). HSV and/or CMV had been detected by two laboratory strategies in 57 examined kids (51%). Of those, in 18 kids (16.2%) each HSV and CMV had been detected.
The coincidence of the outcomes of the detection of HSV and CMV by these two strategies was noticed in 72.4% and 75.2% of circumstances respectively. The comparative evaluation of the detection of anti-CMV IgG and IgM was made with the usage of industrial check techniques produced bythe following producers: “Vector-Finest” and “Bioservice” (Russia), “HUMAN” and “Boehringer” (Germany). The efficient detection of each anti-CMV (IgG and IgM) was ensured by the check techniques “Boehringer”.
The check system “Vector-Finest” for anti-CMV IgG proved to be not inferior as regards sensitivity and specificity. The German check techniques demonstrated the very best specificity within the detection of low-avid antibodies. The info obtained on this examine point out that the detection price of HSV and CMV markers in newborns and infants suspected for herpesvirus an infection was, on the common, 20 – 40%.
Dependable diagnostics in newborns and infants is feasible solely within the presence of the mixture of a minimum of 2 serological exams (the dedication of antivirus IgM and IgG avidity) and a couple of strategies for the detection of direct herpesvirus markers (PCR and RCM).