Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a key instrument for the evaluation of organic tissues. It offers spatial and quantitative details about various kinds of analytes inside tissue sections. Despite the growing enhancements of this method, the low detection sensitivity of some compounds stays an essential problem to beat.
Poor sensitivity is expounded to weak ionization effectivity, low abundance of analytes and matrix ions, or endogenous interferences. On-tissue chemical derivatization (OTCD) has confirmed to be an essential answer to those points and is more and more employed in MALDI MSI research. OTCD reagents, synthesized or commercially accessible, have been primarily used for the detection of small exogenous or endogenous molecules inside tissues.
Optimally, an OTCD response is carried out in delicate situations, in an appropriate vary of time, preserves the integrity of the tissues, and prevents the delocalization. In addition to their reactivity with a focused chemical operate, some OTCD reagents may also be used as a matrix, which simplifies the pattern preparation process. In this assessment, we current an exhaustive overview of OTCD reagents and strategies utilized in MALDI MSI research.

Synthesis of Isothiocyanates Using DMT/NMM/TsO  as a New Desulfurization Reagent

Thirty-three alkyl and aryl isothiocyanates, in addition to isothiocyanate derivatives from esters of coded amino acids and from esters of unnatural amino acids (6-aminocaproic, 4-(aminomethyl)benzoic, and tranexamic acids), have been synthesized with passable or excellent yields (25-97%). Synthesis was carried out in a “one-pot”, two-step process, within the presence of natural base (Et<sub>3</sub>N, DBU or NMM), and carbon disulfide by way of dithiocarbamates, with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TsO<sup>-</sup>) as a desulfurization reagent.
For the synthesis of aliphatic and fragrant isothiocyanates, reactions have been carried out in a microwave reactor, and chosen alkyl isothiocyanates have been additionally synthesized in aqueous medium with excessive yields (72-96%). Isothiocyanate derivatives of L- and D-amino acid methyl esters have been synthesized, underneath situations with out microwave radiation help, with low racemization (er 99 > 1), and their absolute configuration was confirmed by round dichroism.
Isothiocyanate derivatives of pure and unnatural amino acids have been evaluated for antibacterial exercise on <i>E. coli</i> and <i>S. aureus</i> bacterial strains, the place probably the most lively was ITC <b>9e.</b>

Producing molecular biology reagents with out purification

We lately developed ‘mobile’ reagents-lyophilized micro organism overexpressing proteins of interest-that can exchange business pure enzymes in typical diagnostic and molecular biology reactions. To make mobile reagent expertise broadly accessible and amenable to native manufacturing with minimal instrumentation, we now report a considerably simplified methodology for getting ready mobile reagents that requires solely a typical bacterial incubator to develop and subsequently dry enzyme-expressing micro organism at 37°C with the help of cheap chemical desiccants.
We show utility of such dried mobile reagents in frequent molecular and artificial biology processes, akin to PCR, qPCR, reverse transcription, isothermal amplification, and Golden Gate DNA meeting, in constructing easy-to-use testing kits, and in speedy reagent manufacturing for assembly extraordinary diagnostic calls for akin to these being confronted within the ongoing SARS-CoV-2 pandemic.
 On-tissue chemical derivatization reagents for matrix-assisted laser desorption/ionization mass spectrometry imaging
Furthermore, we show feasibility of native manufacturing by efficiently implementing this minimized process and getting ready mobile reagents in a number of international locations, together with the United Kingdom, Cameroon, and Ghana. Our outcomes show potentialities for readily scalable native and distributed reagent manufacturing, and additional instantiate the alternatives accessible by way of artificial biology on the whole.

Difluoroallyl ammonium salts: extremely versatile, steady and selective gem-difluoroallylation reagents

The selective synthesis of fluorinated natural molecules continues to be of main significance for the event of bioactive compounds (agrochemicals and prescribed drugs) in addition to distinctive supplies. Among the established artificial toolbox for incorporation of fluorine-containing items, environment friendly and normal reagents for introducing -CF2– teams have been largely uncared for.
Here, we current the synthesis of three,3-difluoropropen-1-yl ammonium salts (DFPAs) as steady, and scalable gem-difluoromethylation reagents, which permit for the direct response with a variety of fascinating nucleophiles. DFPAs easily react with N-, O-, S-, Se-, and C-nucleophiles underneath delicate situations with out necessity of metallic catalysts with unique regioselectivity. In this manner, the introduced reagents additionally allow the easy preparation of many analogues of current prescribed drugs.

Effect of the Fuel Equivalence Ratio on the Mechanisms of Thiophene Oxidation in Water Vapor at Increased Density of the Reagents

The article presents the outcomes of a examine of thiophene oxidation in high-density C4H4S/O2 mixtures (ρThi = 0.12 and 0.15 mol/dm3, ρO2 = 0.74-1.26 mol/dm3), diluted with water vapor and argon (dilution stage x D = 35-65% mol), at uniform heating (1 Okay/min) of a stainless-steel tubular reactor as much as 823 Okay. It is established that the temperature of thiophene oxidation onset weakly will depend on the character of the diluent and the oxygen content material within the response combination.
From the time dependences of the response mixtures on temperature and stress, it follows that the oxidation of thiophene within the water vapor and argon media proceeds in response to the mechanisms of homogeneous and heterogeneous reactions. Upon oxidation of thiophene within the stoichiometric mixtures in argon with a small quantity of water vapor, in addition to within the lean mixtures in water vapor, the contribution of reactions on the floor of the Pt-Rh/Pt thermocouple, inserted into the middle of the response quantity, is elevated.
Upon oxidation of thiophene in water vapor within the fuel-enriched and stoichiometric mixtures, reactions on the oxidized floor of the reactor wall (primarily iron oxides) prevail. Increasing the density of water vapor each reduces the contribution of heterogeneous reactions on the reactor wall and prevents full carbon burnout.

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OOSA10411-5ML - SERUM:Azide Free Serum & Cells

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OOSA10417-50ML - SERUM:Azide Free Serum & Cells

OOSA10417-50ML Aviva Systems Biology 50ml 469 EUR

OOSA10426-20ML - SERUM:Azide Free Serum & Cells

OOSA10426-20ML Aviva Systems Biology 20ml 99 EUR

OOSA10430-10ML - SERUM:Azide Free Serum & Cells

OOSA10430-10ML Aviva Systems Biology 10ml 379 EUR

OOSA10419-100ML - SERUM:Azide Free Serum & Cells

OOSA10419-100ML Aviva Systems Biology 100ml 749 EUR

OOSA11794-100ML - SERUM:Azide free Serum & Cells

OOSA11794-100ML Aviva Systems Biology 100ml 1489 EUR

PIG SERUM:Azide Free

MBS239301-100mL MyBiosource 100mL 220 EUR

PIG SERUM:Azide Free

MBS239301-5x100mL MyBiosource 5x100mL 805 EUR

OOSA10413-5ML - Serum:Azide Free

OOSA10413-5ML Aviva Systems Biology 5ml 69 EUR

OOSA10415-10ML - SERUM:Azide Free

OOSA10415-10ML Aviva Systems Biology 10ml 139 EUR

OOSA10428-100ML - SERUM:Azide Free

OOSA10428-100ML Aviva Systems Biology 100ml 139 EUR

Bovine Serum Albumin Fatty Acid Free Reagent Grade

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Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

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Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade

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Free Chlorine Reagents 0-5mg/L - EACH

WAT1028 Scientific Laboratory Supplies EACH 49.95 EUR

Albumin, Human Serum (Azide Free) (HSA)

MBS6120697-025mg MyBiosource 0.25(mg 495 EUR

Albumin, Human Serum (Azide Free) (HSA)

MBS6120697-1mg MyBiosource 1mg 1000 EUR

Albumin, Human Serum (Azide Free) (HSA)

MBS6120697-5x1mg MyBiosource 5x1mg 4350 EUR

VEX Exosome Isolation Reagent (from serum)

R602 Vazyme 10 ml 769.2 EUR

Human Serum Albumin Antigen, Reagent Grade

MBS568775-100g MyBiosource 100g 1240 EUR

Human Serum Albumin Antigen, Reagent Grade

MBS568775-5x100g MyBiosource 5x100g 5385 EUR

EZ-DNA Reagents

SK8201 Bio Basic 100preps 112.2 EUR

ALB (ALB, Serum albumin) (Azide free) (HRP)

MBS6121446-02mL MyBiosource 0.2(mL 980 EUR

ALB (ALB, Serum albumin) (Azide free) (HRP)

MBS6121446-5x02mL MyBiosource 5x0.2mL 4250 EUR

Ecl Detection Reagents

RPN3004 Scientific Laboratory Supplies EACH 552.9 EUR

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-100 Biovision each 783.6 EUR

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-25 Biovision each 294 EUR

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-5 Biovision each 144 EUR

T-Pro Total Exosome Isolation reagent (from serum)

JO66-V002M T-Pro Biotechnology 25ml/BT 800 EUR

T-Pro Total Exosome Isolation reagent (from serum)

JO66-V002S T-Pro Biotechnology 1ml*5/set 200 EUR

Albumin, Human Serum (BSA) (BSA & Azide Free)

MBS6120698-025mg MyBiosource 0.25(mg 445 EUR

Albumin, Human Serum (BSA) (BSA & Azide Free)

MBS6120698-1mg MyBiosource 1mg 1015 EUR

Albumin, Human Serum (BSA) (BSA & Azide Free)

MBS6120698-5x1mg MyBiosource 5x1mg 4415 EUR

Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!

P5W13 101Bio 10 rxn 99 EUR

Albumin, Human Serum (BSA and Azide Free) (HSA)

MBS6120696-1mg MyBiosource 1(mg 1055 EUR

Albumin, Human Serum (BSA and Azide Free) (HSA)

MBS6120696-5x1mg MyBiosource 5x1mg 4600 EUR

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-30T Creative Bioarray 30 T Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)

CEIK-044L-50T Creative Bioarray 50 T Ask for price

Ecl Detection Reagents - EACH

RPN2105 Scientific Laboratory Supplies EACH 815.4 EUR

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade

OPEF01351-100G Aviva Systems Biology 100g 1302 EUR

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)

WA-013 Inventbiotech each 135 EUR

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 MiTeGen 1.5 ml ml 37 EUR
Description: CryoProtX Mix Reagents 1.5 mL

Set of 10 Biolipidure Reagents

Biolipidure-set AS ONE International 10mLx10 1820.4 EUR
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

REAGENTS HIGH RANGE AMMONIA - PK25

HI73325 Scientific Laboratory Supplies PK25 55.35 EUR

High Range Ammonia Reagents - EACH

HI9373301 Scientific Laboratory Supplies EACH 130.95 EUR

PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)

MBS6491998-02mL MyBiosource 0.2mL 980 EUR

PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)

MBS6491998-5x02mL MyBiosource 5x0.2mL 4250 EUR

Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99%

65186 Sisco Laboratories 10 Gms 38.4 EUR
Description: Part B

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO MiTeGen 1.5 ml ml 37 EUR
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents

M1980 Tecan Benelux 1 unit 169.8 EUR

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Alkphos Direct Labelling Reagents - EACH

RPN3680 Scientific Laboratory Supplies EACH 915.3 EUR

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 Alpha Diagnostics 1 mg 196.8 EUR

SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP)

MBS6344814-02mL MyBiosource 0.2mL 980 EUR

SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP)

MBS6344814-5x02mL MyBiosource 5x0.2mL 4250 EUR

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)

MBS6335888-02mL MyBiosource 0.2mL 980 EUR

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)

MBS6335888-5x02mL MyBiosource 5x0.2mL 4250 EUR

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)

MBS6335899-02mL MyBiosource 0.2mL 980 EUR

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)

MBS6335899-5x02mL MyBiosource 5x0.2mL 4250 EUR

CRP Free Serum

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CRP Free Serum

MBS318434-50mL MyBiosource 50mL 1115 EUR

CRP Free Serum

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CRP free serum

MBS663102-1Unit MyBiosource 1Unit 875 EUR

CRP free serum

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Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 GenDepot 450ml 118 EUR

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-100 GenDepot 950ml 130 EUR

iN-fect™ In vitro transfection reagents

15081 iNtRON Biotechnology Inc 500 µL 127.27 EUR
Description: Transfection

Pre-Titrated Reagents for Human EGF ELISA

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Pre-Titrated Reagents for Human EGF ELISA

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Pre-Titrated Reagents for Human EGF ELISA

MBS590076-5x10Plates MyBiosource 5x10Plates 3270 EUR

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN Alpha Diagnostics 100 ug 343.2 EUR

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP)

MBS6274207-02mL MyBiosource 0.2mL 980 EUR

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP)

MBS6274207-5x02mL MyBiosource 5x0.2mL 4250 EUR

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10429-10ML Aviva Systems Biology 10ml 279 EUR

Steroid Free Serum

90R-1007 Fitzgerald 50 mL 363 EUR
Description: Steroid Free Human Serum

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

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Liposomal and Non-Liposomal Transfection Reagents

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Liposomal and Non-Liposomal Transfection Reagents

abx298032-20g Abbexa 20 µg 875 EUR

Liposomal and Non-Liposomal Transfection Reagents

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Myoglobin free serum

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Myoglobin free serum

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Pre-Titrated Reagents for Human IL-8 ELISA

MBS590074-10Plates MyBiosource 10Plates 710 EUR

Pre-Titrated Reagents for Human IL-8 ELISA

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Pre-Titrated Reagents for Human IL-8 ELISA

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Pre-Titrated Reagents for Human MCP-1 ELISA

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Pre-Titrated Reagents for Human MCP-1 ELISA

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Pre-Titrated Reagents for Human MCP-1 ELISA

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Pre-Titrated Reagents for Human MCP-3 ELISA

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Pre-Titrated Reagents for Human MCP-3 ELISA

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Pre-Titrated Reagents for Human MCP-3 ELISA

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Pre-Titrated Reagents for Human IL-16 ELISA

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Pre-Titrated Reagents for Human IL-16 ELISA

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Pre-Titrated Reagents for Human IL-16 ELISA

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Pre-Titrated Reagents for Human IL-18 ELISA

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Pre-Titrated Reagents for Human IL-18 ELISA

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Pre-Titrated Reagents for Human IL-18 ELISA

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PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP)

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PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP)

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RAT SERUM WITH 0.09% AZIDE

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RAT SERUM WITH 0.09% AZIDE

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RAT SERUM WITH 0.09% AZIDE

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RAT SERUM WITH 0.09% AZIDE

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Pre-Titrated Reagents for Human GM-CSF ELISA

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Pre-Titrated Reagents for Human GM-CSF ELISA

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Pre-Titrated Reagents for Human GM-CSF ELISA

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Pre-Titrated Reagents for Human TNF-alpha ELISA

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Pre-Titrated Reagents for Human TNF-alpha ELISA

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Pre-Titrated Reagents for Human TNF-alpha ELISA

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Myeloperoxidase Free Serum

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Myeloperoxidase free serum

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Myeloperoxidase free serum

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Troponin I Free Serum

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Troponin I Free Serum

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Troponin I Free Serum

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Cystatin C free serum

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Cystatin C free serum

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Apo AI/B Free Serum

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Human Serum (CRP free)

90R-100 Fitzgerald 100 ml 1326 EUR
Description: C-reactive protein free normal human serum

Human Serum (FABP free)

90R-109 Fitzgerald 100 ml 995 EUR
Description: FABP free normal human serum

Human Serum (FABP free)

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Human Serum (FABP free)

MBS539647-5x100mL MyBiosource 5x100mL 7035 EUR

Serum Free Medium For Rat Neural Stem Cells

RAXNF-90011 Cyagen 100mL 289 EUR

Human Serum (Myoglobin Free)

90R-110 Fitzgerald 100 ml 995 EUR
Description: Myoglobin free normal human serum

Human Serum (Myoglobin Free)

MBS536546-100mL MyBiosource 100mL 1615 EUR

Human Serum (Myoglobin Free)

MBS536546-5x100mL MyBiosource 5x100mL 7035 EUR

HEK 293 Media, Serum Free

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OOMA00062-50ML - CRP Free Serum

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MOUSE SERUM WITH 0.09% AZIDE Serum Products

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MOUSE SERUM WITH 0.09% AZIDE Serum Products

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MOUSE SERUM WITH 0.09% AZIDE Serum Products

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RABBIT SERUM WITH 0.09% AZIDE Serum Products

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RABBIT SERUM WITH 0.09% AZIDE Serum Products

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Serum Free Complete Medium For Rat Fetal Neuron

RAXFN-90011 Cyagen 100mL 250 EUR

RANGE 13 - Ventilated cabinet for reagents and toxic products - 250 L

MF4V Ecosafe Sa each 2377.76 EUR

RANGE 13 - Cabinet for reagents and toxic products (without fan) - 250 L

MF4SV. Ecosafe Sa each 2158.24 EUR
It is proven that the neutralization of sulfuric acid, ensuing from the oxidation of thiophene, with calcium carbonate reduces the corrosion of stainless-steel. The X-ray diffraction evaluation revealed the presence of ferrochromite, iron and chromium oxides, iron, nickel, and chromium sulfates within the corrosion merchandise.

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